Evaluation of ELISA and IHAT in serological diagnosis of proven cases of human hydatidosis

Cystic hydatid disease [Hydatidosis] is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. Hydatidosis in man [as an accidental host] is caused by infection with the ova containing larval stage of Echinococcus granulosus. In the last decade, different techniques have been employed for sero-diagnosis of hydatidosis; as IHA, IFA, ELISA, CCLE [Counter Current Immuno-electrophoresis]. This paper evaluated the validity of ELISA and IHA. Since whole hydatid cyst fluid was used as a source of antigen for serodiagnosis. Thirty surgical and pathological hydatidosis proven patients were examined. The sensitivity and specificity of ELISA were 96.7% and 97.5% respectively, and that of IRA were 86.7%, and 95% respectively

indirect immunological effect of concurrent human schistosomiasis and fascioliasis on helicobacter pylori pathogenesis

The impact of Schistosoma mansoni and Fasciola parasitic infections on H. pylori induced pathogenesis was studied in 20 patients exclusively having H. pylori infection alone. The patients were compared to 20 patients having schistosomiasis mansoni co-infected with H. pylori and to another 20 patients having fascioliasis co-infected with H. pylori. 15 patients exclusively having schistosomiasis mansoni alone and 15 patients exclusively having fascioliasis alone were also included in the study. All patients were subjected to thorough medical examination, parasitological, endoscopic and histopathological studies. The results showed that severe gastritis was significantly more common in the patients exclusively infected with H. pylori alone. Chronic atrophic gastritis and intestinal metaplasia or precancerous lesions were present in patients with exclusively H. pylori infection. We may conclude that the concomitant of parasitic diseases with H. pylori infection probably reduce the gastroduodenal pathogenesis through indirect immune response